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Cloning and Expression
Enzymes
AvrII
Supplier Images
AvrII
AvrII from New England Biolabs
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Citations:
(25)
Supplier Page
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New England Biolabs for
AvrII
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Company Info
Citations
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Product Specs
Item
AvrII
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0174S
Quantity
100 units
Concentration
4 u/µL
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
C/CTAGG
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (25)
(21)
Noncanonical mechanism of voltage sensor coupling to pore revealed by tandem dimers of Shaker
Nature Communications
December 1, 2019
João L. Carvalho-de-Souza, Francisco Bezanilla
eliminate the stop codon and introduce a unique restriction site for digestion with
AvrII
endonuclease (#
R0174L
New England BioLabs
, Ipswich, MA; see primers in Supplementary Note 4). Other additional mutations in
(22)
Neurocalcin regulates nighttime sleep and arousal in Drosophila
eLife
March 13, 2019
Ko-Fan Chen, Simon Lowe, Angélique Lamaze, Patrick Krätschmer, James Jepson
ThermoFisher Scientific
), and subsequently sub-cloned via
NotI
(
R3189S
,
NEB
) and
AscI
/
AvrII
digestion (
R0558S
and
R0174S
,
NEB
) and T4
ligation
(
M0202S
,
NEB
) into the pTVcherry vector, a P-element construct containing the
(23)
CBD-1 organizes two independent complexes required for eggshell vitelline layer formation and egg activation in C. elegans
Developmental Biology
October 1, 2018
Delfina P. González, Helen V. Lamb, Diana Partida, Zachary T. Wilson, Marie-Claire Harrison, Julián A. Prieto, James J. Moresco, Jolene K. Diedrich, John R. Yates, Sara K. Olson
sources for the vector backbone, perm-2 or perm-4, and mCherry sequences, respectively.
AvrII
(
NEB
,
R0174
) sites were introduced to facilitate
subcloning
from pBluescript into pCFJ151 that targets a Mos1 transposon
(24)
Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies
mAbs
August 1, 2018
Emanuele Sasso et al.
(
R0199
) all from
New England Biolabs
, linearized pEU8.2VH vector, and the VLs in
ApaLI
(
R0507
) and
AvrII
(
R0174
)
New England Biolabs
, linearized pEU4.2VL vector. Stellar Competent Cells (Clontech Laboratories, 636,763)
(25)
Design of Hybrid RNA Polymerase III Promoters for Efficient CRISPR-Cas9 Function
BIO-PROTOCOL
March 20, 2018
Joshua Misa, Cory Schwartz, Ian Wheeldon
(
New England Biolabs
, catalog number:
B7204S
) 17.
AvrII
restriction enzyme (
New England Biolabs
, catalog number:
R0174S
) 18. Taq DNA polymerase (
New England Biolabs
, catalog number:
M0273L
) 19. Yeast extract (BD, DifcoTM,
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AvrII from New England Biolabs
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